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prc cnn3 mgfp plasmid  (Addgene inc)


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    Structured Review

    Addgene inc prc cnn3 mgfp plasmid
    (A–D) Projections of all x-y optical slices of A431 cells stained for (A) E-cadherin (Ecad, red) and F-actin (actin, green); (B) Ecad (green), F-actin (red), and myosin IIA (myosin II, blue); (C) Ecad (green), actin, and calponin-3 <t>(Cnn3);</t> and (D) Cnn3 (green) and myosin IIA (red).
    Prc Cnn3 Mgfp Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/prc+cnn3+mgfp+plasmid/pPAmCherry-b-actin+(Plasmid+%2331949)/pmc07093076-565-15-11
    Average 90 stars, based on 3 article reviews
    prc cnn3 mgfp plasmid - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "Sensing Actin Dynamics through Adherens Junctions"

    Article Title: Sensing Actin Dynamics through Adherens Junctions

    Journal: Cell reports

    doi: 10.1016/j.celrep.2020.01.106

    (A–D) Projections of all x-y optical slices of A431 cells stained for (A) E-cadherin (Ecad, red) and F-actin (actin, green); (B) Ecad (green), F-actin (red), and myosin IIA (myosin II, blue); (C) Ecad (green), actin, and calponin-3 (Cnn3); and (D) Cnn3 (green) and myosin IIA (red).
    Figure Legend Snippet: (A–D) Projections of all x-y optical slices of A431 cells stained for (A) E-cadherin (Ecad, red) and F-actin (actin, green); (B) Ecad (green), F-actin (red), and myosin IIA (myosin II, blue); (C) Ecad (green), actin, and calponin-3 (Cnn3); and (D) Cnn3 (green) and myosin IIA (red).

    Techniques Used: Staining

    (A–D) Projections of all x-y optical slices of Cnn3-Dn-expressing A431 cells imaged for Dendra2 fluorescence (green, Cnn3-Dn) and stained for (A) Ecad, (B) actin, (C) vinculin (Vcn), and (D) mena. Scale bar, 10 μm. The zoomed areas (indicated by dashed boxes in Cnn3-Dn images) are presented at the bottom. Scale bar, 5 μm. Arrowheads indicate the gaps in Cnn3-Dn distribution along pAJ-linked bundles that are precisely matched by the antibody staining.
    Figure Legend Snippet: (A–D) Projections of all x-y optical slices of Cnn3-Dn-expressing A431 cells imaged for Dendra2 fluorescence (green, Cnn3-Dn) and stained for (A) Ecad, (B) actin, (C) vinculin (Vcn), and (D) mena. Scale bar, 10 μm. The zoomed areas (indicated by dashed boxes in Cnn3-Dn images) are presented at the bottom. Scale bar, 5 μm. Arrowheads indicate the gaps in Cnn3-Dn distribution along pAJ-linked bundles that are precisely matched by the antibody staining.

    Techniques Used: Expressing, Fluorescence, Staining

    (A–C) Maximum projection view of control A431-EcGFP cells (A431-EcGFP) or after Cfl1-KO (A431-EcGFP-Cfl1-KO). Cells were imaged for GFP (EcGFP) and for (A) Cfl1 (Cfl, red), (B) actin (actin, red), and (C) Cnn-3 (Cnn3, red). Scale bar, 10 μm. The zoomed areas (indicated by dashed boxes in EcGFP images) are presented in the insets (A) or at the bottom (B and C). Scale bar, 5 μm.
    Figure Legend Snippet: (A–C) Maximum projection view of control A431-EcGFP cells (A431-EcGFP) or after Cfl1-KO (A431-EcGFP-Cfl1-KO). Cells were imaged for GFP (EcGFP) and for (A) Cfl1 (Cfl, red), (B) actin (actin, red), and (C) Cnn-3 (Cnn3, red). Scale bar, 10 μm. The zoomed areas (indicated by dashed boxes in EcGFP images) are presented in the insets (A) or at the bottom (B and C). Scale bar, 5 μm.

    Techniques Used:

    KEY RESOURCES TABLE
    Figure Legend Snippet: KEY RESOURCES TABLE

    Techniques Used: Recombinant, CRISPR, Plasmid Preparation, Software

    Related Articles

    Plasmid Preparation:

    Article Title: Sensing Actin Dynamics through Adherens Junctions
    Article Snippet: .. The plasmid encoding PA-mCH-tagged b-actin was a gift from Dr. Verkhusha (Addgene, plasmid # 31949). pRc-CNN3-mGFP plasmid was generated by using Myc-DDK tagged CNN3 (Origene, RC200488). ..

    Generated:

    Article Title: Sensing Actin Dynamics through Adherens Junctions
    Article Snippet: .. The plasmid encoding PA-mCH-tagged b-actin was a gift from Dr. Verkhusha (Addgene, plasmid # 31949). pRc-CNN3-mGFP plasmid was generated by using Myc-DDK tagged CNN3 (Origene, RC200488). ..



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    (A–D) Projections of all x-y optical slices of A431 cells stained for (A) E-cadherin (Ecad, red) and F-actin (actin, green); (B) Ecad (green), F-actin (red), and myosin IIA (myosin II, blue); (C) Ecad (green), actin, and calponin-3 <t>(Cnn3);</t> and (D) Cnn3 (green) and myosin IIA (red).
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    prc cnn3 mgfp plasmid  (Addgene inc)
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    (A–D) Projections of all x-y optical slices of A431 cells stained for (A) E-cadherin (Ecad, red) and F-actin (actin, green); (B) Ecad (green), F-actin (red), and myosin IIA (myosin II, blue); (C) Ecad (green), actin, and calponin-3 <t>(Cnn3);</t> and (D) Cnn3 (green) and myosin IIA (red).
    Prc Cnn3 Mgfp Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/prc+cnn3+mgfp+plasmid/pPAmCherry-b-actin+(Plasmid+%2331949)/pmc07093076-565-15-11
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    Image Search Results


    (A–D) Projections of all x-y optical slices of A431 cells stained for (A) E-cadherin (Ecad, red) and F-actin (actin, green); (B) Ecad (green), F-actin (red), and myosin IIA (myosin II, blue); (C) Ecad (green), actin, and calponin-3 (Cnn3); and (D) Cnn3 (green) and myosin IIA (red).

    Journal: Cell reports

    Article Title: Sensing Actin Dynamics through Adherens Junctions

    doi: 10.1016/j.celrep.2020.01.106

    Figure Lengend Snippet: (A–D) Projections of all x-y optical slices of A431 cells stained for (A) E-cadherin (Ecad, red) and F-actin (actin, green); (B) Ecad (green), F-actin (red), and myosin IIA (myosin II, blue); (C) Ecad (green), actin, and calponin-3 (Cnn3); and (D) Cnn3 (green) and myosin IIA (red).

    Article Snippet: The plasmid encoding PA-mCH-tagged b-actin was a gift from Dr. Verkhusha (Addgene, plasmid # 31949). pRc-CNN3-mGFP plasmid was generated by using Myc-DDK tagged CNN3 (Origene, RC200488).

    Techniques: Staining

    (A–D) Projections of all x-y optical slices of Cnn3-Dn-expressing A431 cells imaged for Dendra2 fluorescence (green, Cnn3-Dn) and stained for (A) Ecad, (B) actin, (C) vinculin (Vcn), and (D) mena. Scale bar, 10 μm. The zoomed areas (indicated by dashed boxes in Cnn3-Dn images) are presented at the bottom. Scale bar, 5 μm. Arrowheads indicate the gaps in Cnn3-Dn distribution along pAJ-linked bundles that are precisely matched by the antibody staining.

    Journal: Cell reports

    Article Title: Sensing Actin Dynamics through Adherens Junctions

    doi: 10.1016/j.celrep.2020.01.106

    Figure Lengend Snippet: (A–D) Projections of all x-y optical slices of Cnn3-Dn-expressing A431 cells imaged for Dendra2 fluorescence (green, Cnn3-Dn) and stained for (A) Ecad, (B) actin, (C) vinculin (Vcn), and (D) mena. Scale bar, 10 μm. The zoomed areas (indicated by dashed boxes in Cnn3-Dn images) are presented at the bottom. Scale bar, 5 μm. Arrowheads indicate the gaps in Cnn3-Dn distribution along pAJ-linked bundles that are precisely matched by the antibody staining.

    Article Snippet: The plasmid encoding PA-mCH-tagged b-actin was a gift from Dr. Verkhusha (Addgene, plasmid # 31949). pRc-CNN3-mGFP plasmid was generated by using Myc-DDK tagged CNN3 (Origene, RC200488).

    Techniques: Expressing, Fluorescence, Staining

    (A–C) Maximum projection view of control A431-EcGFP cells (A431-EcGFP) or after Cfl1-KO (A431-EcGFP-Cfl1-KO). Cells were imaged for GFP (EcGFP) and for (A) Cfl1 (Cfl, red), (B) actin (actin, red), and (C) Cnn-3 (Cnn3, red). Scale bar, 10 μm. The zoomed areas (indicated by dashed boxes in EcGFP images) are presented in the insets (A) or at the bottom (B and C). Scale bar, 5 μm.

    Journal: Cell reports

    Article Title: Sensing Actin Dynamics through Adherens Junctions

    doi: 10.1016/j.celrep.2020.01.106

    Figure Lengend Snippet: (A–C) Maximum projection view of control A431-EcGFP cells (A431-EcGFP) or after Cfl1-KO (A431-EcGFP-Cfl1-KO). Cells were imaged for GFP (EcGFP) and for (A) Cfl1 (Cfl, red), (B) actin (actin, red), and (C) Cnn-3 (Cnn3, red). Scale bar, 10 μm. The zoomed areas (indicated by dashed boxes in EcGFP images) are presented in the insets (A) or at the bottom (B and C). Scale bar, 5 μm.

    Article Snippet: The plasmid encoding PA-mCH-tagged b-actin was a gift from Dr. Verkhusha (Addgene, plasmid # 31949). pRc-CNN3-mGFP plasmid was generated by using Myc-DDK tagged CNN3 (Origene, RC200488).

    Techniques:

    KEY RESOURCES TABLE

    Journal: Cell reports

    Article Title: Sensing Actin Dynamics through Adherens Junctions

    doi: 10.1016/j.celrep.2020.01.106

    Figure Lengend Snippet: KEY RESOURCES TABLE

    Article Snippet: The plasmid encoding PA-mCH-tagged b-actin was a gift from Dr. Verkhusha (Addgene, plasmid # 31949). pRc-CNN3-mGFP plasmid was generated by using Myc-DDK tagged CNN3 (Origene, RC200488).

    Techniques: Recombinant, CRISPR, Plasmid Preparation, Software

    (A–D) Projections of all x-y optical slices of A431 cells stained for (A) E-cadherin (Ecad, red) and F-actin (actin, green); (B) Ecad (green), F-actin (red), and myosin IIA (myosin II, blue); (C) Ecad (green), actin, and calponin-3 (Cnn3); and (D) Cnn3 (green) and myosin IIA (red).

    Journal: Cell reports

    Article Title: Sensing Actin Dynamics through Adherens Junctions

    doi: 10.1016/j.celrep.2020.01.106

    Figure Lengend Snippet: (A–D) Projections of all x-y optical slices of A431 cells stained for (A) E-cadherin (Ecad, red) and F-actin (actin, green); (B) Ecad (green), F-actin (red), and myosin IIA (myosin II, blue); (C) Ecad (green), actin, and calponin-3 (Cnn3); and (D) Cnn3 (green) and myosin IIA (red).

    Article Snippet: The plasmid encoding PA-mCH-tagged b-actin was a gift from Dr. Verkhusha (Addgene, plasmid # 31949). pRc-CNN3-mGFP plasmid was generated by using Myc-DDK tagged CNN3 (Origene, RC200488).

    Techniques: Staining

    (A–D) Projections of all x-y optical slices of Cnn3-Dn-expressing A431 cells imaged for Dendra2 fluorescence (green, Cnn3-Dn) and stained for (A) Ecad, (B) actin, (C) vinculin (Vcn), and (D) mena. Scale bar, 10 μm. The zoomed areas (indicated by dashed boxes in Cnn3-Dn images) are presented at the bottom. Scale bar, 5 μm. Arrowheads indicate the gaps in Cnn3-Dn distribution along pAJ-linked bundles that are precisely matched by the antibody staining.

    Journal: Cell reports

    Article Title: Sensing Actin Dynamics through Adherens Junctions

    doi: 10.1016/j.celrep.2020.01.106

    Figure Lengend Snippet: (A–D) Projections of all x-y optical slices of Cnn3-Dn-expressing A431 cells imaged for Dendra2 fluorescence (green, Cnn3-Dn) and stained for (A) Ecad, (B) actin, (C) vinculin (Vcn), and (D) mena. Scale bar, 10 μm. The zoomed areas (indicated by dashed boxes in Cnn3-Dn images) are presented at the bottom. Scale bar, 5 μm. Arrowheads indicate the gaps in Cnn3-Dn distribution along pAJ-linked bundles that are precisely matched by the antibody staining.

    Article Snippet: The plasmid encoding PA-mCH-tagged b-actin was a gift from Dr. Verkhusha (Addgene, plasmid # 31949). pRc-CNN3-mGFP plasmid was generated by using Myc-DDK tagged CNN3 (Origene, RC200488).

    Techniques: Expressing, Fluorescence, Staining

    (A–C) Maximum projection view of control A431-EcGFP cells (A431-EcGFP) or after Cfl1-KO (A431-EcGFP-Cfl1-KO). Cells were imaged for GFP (EcGFP) and for (A) Cfl1 (Cfl, red), (B) actin (actin, red), and (C) Cnn-3 (Cnn3, red). Scale bar, 10 μm. The zoomed areas (indicated by dashed boxes in EcGFP images) are presented in the insets (A) or at the bottom (B and C). Scale bar, 5 μm.

    Journal: Cell reports

    Article Title: Sensing Actin Dynamics through Adherens Junctions

    doi: 10.1016/j.celrep.2020.01.106

    Figure Lengend Snippet: (A–C) Maximum projection view of control A431-EcGFP cells (A431-EcGFP) or after Cfl1-KO (A431-EcGFP-Cfl1-KO). Cells were imaged for GFP (EcGFP) and for (A) Cfl1 (Cfl, red), (B) actin (actin, red), and (C) Cnn-3 (Cnn3, red). Scale bar, 10 μm. The zoomed areas (indicated by dashed boxes in EcGFP images) are presented in the insets (A) or at the bottom (B and C). Scale bar, 5 μm.

    Article Snippet: The plasmid encoding PA-mCH-tagged b-actin was a gift from Dr. Verkhusha (Addgene, plasmid # 31949). pRc-CNN3-mGFP plasmid was generated by using Myc-DDK tagged CNN3 (Origene, RC200488).

    Techniques:

    KEY RESOURCES TABLE

    Journal: Cell reports

    Article Title: Sensing Actin Dynamics through Adherens Junctions

    doi: 10.1016/j.celrep.2020.01.106

    Figure Lengend Snippet: KEY RESOURCES TABLE

    Article Snippet: The plasmid encoding PA-mCH-tagged b-actin was a gift from Dr. Verkhusha (Addgene, plasmid # 31949). pRc-CNN3-mGFP plasmid was generated by using Myc-DDK tagged CNN3 (Origene, RC200488).

    Techniques: Recombinant, CRISPR, Plasmid Preparation, Software